Cloves leaf oil was isolation from cloves leaf using steam distillation step 1. Eugenol is used in germicides, perfumes, and mouthwashes, in. Eugenol is used in perfumery, for the manufacture of synthetic vanillin, as a dental topical analgesic, and as an antiseptic. Isolation of eugenol report ramsey padilla jane erwin. Eugenol is the main ingredient of the essential oil that can be isolated from the spice clove. Characterization of the isolated clove oil eugenol contains a carbon. Polyeugenol from eugenol of clove leaf oil resulted from redistillation, extraction, and fractionation ektsraksi in solid form yield. Extraction of eugenol from cloves lesson plan for chemistry educators by dean antic 11. Pdf optimization of eugenol extraction from clove oil. Pdf supercritical co2 extraction of essential oil from. To perform this experiment cloves and water were mixed together and heated by a heating mantle in a roundbottom flask. Conclusions the optimum conditions of temperature extraction reactive process that was 40oc, reaction time of 30 minutes, 0.
To isolate acetyleugenol from clove seed and characterize it using ir. It is a pale yellow oily compound that extracted from essential oil especially from. Clove syzygium aromaticum is one of the most valuable spices that has been used for centuries as food preservative and for many medicinal purposes. In the extraction and isolation process of eugenol, firstly essential oil is extracted from the plants.
Hplc uv method development and quantification of eugenol from methanolic extracts of some spices farhin inam, sujata deo, neha narkhede 97 table 1. Synthesis of methyl eugenol from crude cloves leaf oil. Eugenol is used as a component of several dental materials e. Saponification is the process of making soaps from fats and a base. Lab report isolation of eugenol cloves by distilation. Crouse and others published microwave extraction of eugenol from cloves. Essential oil of cloves syzygium aromaticum is one of. Eugenol microemulsion with a concentration of in the isolation of eugenol from clove oil yield of separating the two layers and measuring the volume of the aqueous layer. Isolation of eugenol from freshly ground cloves postlab. The isolation and biological testing of eugenol from clove oil.
Specifically, an unmodified household espresso machine is used to extract the essential oil components of cloves eugenol and acetyleugenol. The main process occured in the eugenol production was saphonification and neutralization processes. Isolation of eugenol from cloves by steam distillation and. In this paper, the commonly used steam distillation procedure has been modified to use microwave extraction. The results of the study showed that the methyl eugenol yield is 99. To characterize eugenol and natural product by gas chromatography spectroscopy gcms and ir spectroscopy. Eugenol, c 10 h 12 o 2 is a one of the compound of phenylpropanoid family. Isolation of eugenol from cloves by julia b on prezi. An experiment for the undergraduate organic chemistry laboratory involving a method for the isolation of eugenol from cloves has been developed. The composition, structure, sources, and applications of. Extraction of eugenol from cloves fisher scientific. The experiment employs microwaveassisted extraction mae of the essential oil from whole cloves using aqueous ethanol and the subsequent isolation of eugenol by acidbase extraction.
They are reported to be widely used in dentistry as temporary filing materials, cavity liners for pulp protection, capping materials, temporary cementation of fixed protheses, impression. Cloves are strongly pungent owing to eugenol, which is extracted by distillation to yield oil of cloves. Lab report 3simple, fractional, steam distillation lab. This week we will use steam distillation to isolate clove oil eugenol from cloves and determine how effective the technique is. Is steam distillation an effective way to extract eugenol from cloves. A mixture of the essential oils, eugenol and acetyleugenol, will be steam distilled from cloves. The extraction of eugenol from cloves is a frequent experiment in organic chemistry labs at the sophomore level. Steam distillation is completed by the distillation of water and another liquid 1. Eugenol can be synthesized into 3 3,4dimetoksifenil propanal as the raw material synthesis of derivatives of antibiotic c research on the isolation of eugenol from clove oil also has been done. The mae allows for extraction of the essential oil in a fraction. Cloves distinctive taste is said to carry the flavor of eugenol 5, which makes sense given the consistently high concentration of eugenol in cloves, which is on the order of 7289% in the extract 67. Oil is often a water immiscible liquid that is separated by distillation.
This plant represents one of the richest source of phenolic compounds such as eugenol, eugenol acetate and. Isolation of eugenol from cloves name justin arthur student lab partner witness 1. This oil is used to prepare microscopic slides for viewing and is also a local anesthetic for toothaches. Oh california state polytechnic university, pomona och dr. With variable temperature 90 0c, 0c, and 0c with full reflux the result obtained can be used to determine the optimum condition of eugenol isolation process from leaf oil clove from vacuum distillation with the largest eugenol volume is 39 ml, the largest weigh is 1. Introduction essential oils are the volatile components associated with the aromas of many plants. The apparatus sketched below was assembled and charged with 1. As indicated in figure 2, the water extract of cloves had no cytotoxic activity on the mcf7 cancer cells. With variable temperature 90 0c, 0c, and 0c with full reflux the result obtained can be used to determine the optimum condition of eugenol isolation process from leaf oil clove from vacuum distillation with the largest eugenol volume is. This laboratory experiment describes the extraction of natural products from plant material in line with recent research advances in this field. The cloves were soaked for about 15 min until thoroughly wetted. Eugenol extraction from cloves using steam distillation. To isolate eugenol from tea through distillation and rotary evaporation learn how to dry liquidsorganic solutions using a drying agent identify relevant parts of the structure of eugenol that result in its classification prelaboratory work.
The cloves that we eat come from the flower part of an evergreen tree, syzygium aromaticum, native to southeast asia 8. Eugenol extraction from cloves using steam distillation the goal of. The objective of this research was to obtain optimum condition of eugenol production from clove oil using a central composite design method. Isolation of eugenol v2 westfield state university. The allylic chain gives eugenol its characteristic strong odor. In a typical solvent extraction process of eugenol from clove, the clove buds are ground and wrapped in filter paper followed by subjecting the.
Isolation of eugenol essential oil from clove buds using steam distillation essay example for free newyorkessays database with more than 65000 college essays for studying. Isolation of eugenol from cloves abstract the experiment conducted, isolation of eugenol from cloves, is the extraction of the essential oil. Extraction of eugenol from cloves thermo fisher scientific. The optimized conditions that provided the optimum yield of eugenol were a sample size of 20 g of clove powder of particle diameter 0. In broad terms, discuss how large a component of the mixture eugenol is. Methyl eugenol was synthesis from eugenol from step 2 using dimethyl sulphate step 3. Steam distillation of a volatile oil from cloves purpose. Extraction of eugenol from cloves thermo scientific. Steam distillation separation process for organic aromatic compounds degrade decompose at higher temperatures steam distillation allows the extraction to run at a lower temperature so the extracted compound wont decompose as the water boils, eugenol is trapped within its vapors. Isolation of eugenol report ramsey padilla jane erwin 03292012 introduction.
As a conclusion, this method is extremely simple and inexpensive for isolation of methyl eugenol from cloves leaf oil. The boiling point of eugenol, an oil found in cloves, is 248 c, but it can be isolated at a lower temperature by performing a codistillation with water, this process is. Microwave extraction of eugenol from cloves world journal of. Eugenol is a substituted methoxy phenol compound, a compound structurally similar to vanillin, but with an allylic functional group in place of the aldehyde in the. Extraction of eugenol from cloves using an unmodified. Eugenol was synthesis from cloves leaf oil using sodium hydroxide naoh 5% and sulphuric acid h2so4 5% at ph 3 step 2. Eugenol has wideranging applications, from dental materials to food additives. Isolation of eugenol from cloves cloves water organic alayer a distill b extract wch 2cl 2 3 times e add c onc. Isolation of eugenol from cloves lab report free essays. Hcl discare aqueous layer combined organic layers disare distillation flask clove residue collection flask distillate eugenol eug. Chem 424 lab projects unknowns 1 eugenol extraction from. Lab report 6 isolation of eugenol from cloves by distillation free. Isolation and characterization of eugenol from cloves.
Microwaveassisted isolation of eugenol from cloves. The isolation of eugenol from cloves purpose to perform a steam distillation using a microscale distillation apparatus and isolate a natural product from cloves. Background the boiling point of eugenol, an oil found in cloves, is 248 c, but it can be isolated at a. From the gcadd figure number and gcmsadd figure numbers the clove oil was found to be composed of eugenol 86%, caryophyllene 7% and eugenyl acetate 7%. A greener undergraduate experiment for the organic chemistry lab find, read and cite. This straightforward and uncomplicated method expedites the extraction of clove oil and requires simple and. Reactive extraction process in isolation of eugenol of clove.
Isolation of oil of clove and separation of eugenol and. Results and discussion the raw material was the essential oil of cloves eugenol its concentration was analyzed by gc and found concentrations of eugenol distillate which was then measured its volume and analyzed. Scientific and common names of spices to be studied common name scientific name family used parts cloves syzygium aromaticum myrtaceae bud dalchini cinnamomum zeylanicum lauraceae bark. Various extraction methods have been practiced globally for the. The clove is the bud of an east indian evergreen tree. Steam distillation allows mixtures of organic substances to distill and form a compound known as a distillate. Essay about extraction of eugenol from cloves oil 994. To isolate eugenol and neutral product from cloves by using steam distillation.
Juniper tar oil, a yellow oil obtained through distillation and used in soaps, ointments, and pharmaceuticals. Afterwards, the essential oils are mixed with 3% solution of sodium or potassium hydroxide for the extraction of eugenol. Isolation of eugenol from cloves by steam distillation and its. For example, the derivative of eugenol, acetyl eugenol itself play important role in food flavorings. Eugenol, the main ingredient in clove oil, is a familiar fragrance in many dental offices as it is often mixed into a paste and used in dentistry as a local antiseptic. These compounds are isolated from aqueous distillate by extraction into dichloromethane. You will need to acquire an ir, hnmr, cnmr and gcms and provide a. Methyl eugenol produced from this research according to market needs with a minimum purity of 98.
To purify eugenol from clove leaves, analyze purity with gc, and obtain a percent yield. Eugenol is also found in bay leaves and allspice, and other botanical oil, but in lower concentrations. Because cloves showed some promising results during bslt, further testing was done on the clove extract and the essential oil by using an mtt assay to evaluate the cytotoxic potential of cloves on mcf7 cells. A direct stem distillation took place, and the oil product was out aside for a week.
Extraction and isolation of eugenol the most common method for the isolation of eugenol is steam distillation. The spices cloves, dalchini, tejpatta,nutmeg and tulsi all of them contain an active component eugenol which can be helpful in preparation of various medicines. A greener undergraduate experiment for the organic chemistry lab. Isolation of eugenol from cloves an inquisitive lab demonstration duration. In this study, an hptlc densitometric method was developed for the determination of eugenol in above mentioned five spices as cloves dalchini,tejpatta,nutmeg and tulsi. Prominent sources of eugenol are clove, cinnamon, tulsi and pepper. The most common method for the isolation of eugenol is steam distillation. Isolation of methyl eugenol from cloves leaf oil using acid and based chemicals reactions has been done. This change resulted in a simplified procedure, a reduction in the amount of sample required, and an increase in the efficiency of the extraction of eugenol from dried cloves. Such products are principally combinations of zinc oxide and eugenol in varying ratios. The majority of cloves are used by kretek cigarette manufacturers in indonesia and only about 10 % for other purposes such as folk medicine, food flavouring, food preservatives, fragrance and pharmaceuticals. These functional groups provide the basis for simple chemical tests used to characterize the clove oil. Clove is native of indonesia but nowadays is cultured in several parts of the world including brazil in the state of bahia.
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