Lab report 3simple, fractional, steam distillation lab. A greener undergraduate experiment for the organic chemistry lab find, read and cite. Isolation of eugenol from cloves by steam distillation and its. The composition, structure, sources, and applications of. Eugenol has wideranging applications, from dental materials to food additives. The optimized conditions that provided the optimum yield of eugenol were a sample size of 20 g of clove powder of particle diameter 0. Polyeugenol from eugenol of clove leaf oil resulted from redistillation, extraction, and fractionation ektsraksi in solid form yield. Clove syzygium aromaticum is one of the most valuable spices that has been used for centuries as food preservative and for many medicinal purposes.
To isolate eugenol and neutral product from cloves by using steam distillation. Steam distillation is completed by the distillation of water and another liquid 1. Because cloves showed some promising results during bslt, further testing was done on the clove extract and the essential oil by using an mtt assay to evaluate the cytotoxic potential of cloves on mcf7 cells. Methyl eugenol was synthesis from eugenol from step 2 using dimethyl sulphate step 3. Isolation of eugenol essential oil from clove buds using steam distillation essay example for free newyorkessays database with more than 65000 college essays for studying. Isolation of eugenol from freshly ground cloves postlab.
This change resulted in a simplified procedure, a reduction in the amount of sample required, and an increase in the efficiency of the extraction of eugenol from dried cloves. The cloves were soaked for about 15 min until thoroughly wetted. Isolation of eugenol report ramsey padilla jane erwin. Isolation of eugenol v2 westfield state university. The results of the study showed that the methyl eugenol yield is 99. An experiment for the undergraduate organic chemistry laboratory involving a method for the isolation of eugenol from cloves has been developed. Juniper tar oil, a yellow oil obtained through distillation and used in soaps, ointments, and pharmaceuticals. It is a pale yellow oily compound that extracted from essential oil especially from.
This oil is used to prepare microscopic slides for viewing and is also a local anesthetic for toothaches. Steam distillation separation process for organic aromatic compounds degrade decompose at higher temperatures steam distillation allows the extraction to run at a lower temperature so the extracted compound wont decompose as the water boils, eugenol is trapped within its vapors. Isolation and characterization of eugenol from cloves. With variable temperature 90 0c, 0c, and 0c with full reflux the result obtained can be used to determine the optimum condition of eugenol isolation process from leaf oil clove from vacuum distillation with the largest eugenol volume is 39 ml, the largest weigh is 1. The cloves that we eat come from the flower part of an evergreen tree, syzygium aromaticum, native to southeast asia 8. In broad terms, discuss how large a component of the mixture eugenol is. Eugenol is also found in bay leaves and allspice, and other botanical oil, but in lower concentrations. Eugenol is used as a component of several dental materials e. They are reported to be widely used in dentistry as temporary filing materials, cavity liners for pulp protection, capping materials, temporary cementation of fixed protheses, impression. Extraction of eugenol from cloves lesson plan for chemistry educators by dean antic 11. Is steam distillation an effective way to extract eugenol from cloves. Chem 424 lab projects unknowns 1 eugenol extraction from. The main process occured in the eugenol production was saphonification and neutralization processes.
The objective of this research was to obtain optimum condition of eugenol production from clove oil using a central composite design method. As indicated in figure 2, the water extract of cloves had no cytotoxic activity on the mcf7 cancer cells. In the extraction and isolation process of eugenol, firstly essential oil is extracted from the plants. This laboratory experiment describes the extraction of natural products from plant material in line with recent research advances in this field. Results and discussion the raw material was the essential oil of cloves eugenol its concentration was analyzed by gc and found concentrations of eugenol distillate which was then measured its volume and analyzed. Pdf optimization of eugenol extraction from clove oil. Essential oil of cloves syzygium aromaticum is one of. From the gcadd figure number and gcmsadd figure numbers the clove oil was found to be composed of eugenol 86%, caryophyllene 7% and eugenyl acetate 7%. The isolation of eugenol from cloves purpose to perform a steam distillation using a microscale distillation apparatus and isolate a natural product from cloves. Eugenol microemulsion with a concentration of in the isolation of eugenol from clove oil yield of separating the two layers and measuring the volume of the aqueous layer. Crouse and others published microwave extraction of eugenol from cloves. Extraction of eugenol from cloves fisher scientific. This straightforward and uncomplicated method expedites the extraction of clove oil and requires simple and.
Extraction of eugenol from cloves thermo fisher scientific. The clove is the bud of an east indian evergreen tree. Such products are principally combinations of zinc oxide and eugenol in varying ratios. Oh california state polytechnic university, pomona och dr. Lab report isolation of eugenol cloves by distilation. Eugenol can be synthesized into 3 3,4dimetoksifenil propanal as the raw material synthesis of derivatives of antibiotic c research on the isolation of eugenol from clove oil also has been done. You will need to acquire an ir, hnmr, cnmr and gcms and provide a. Synthesis of methyl eugenol from crude cloves leaf oil. The apparatus sketched below was assembled and charged with 1. With variable temperature 90 0c, 0c, and 0c with full reflux the result obtained can be used to determine the optimum condition of eugenol isolation process from leaf oil clove from vacuum distillation with the largest eugenol volume is. Conclusions the optimum conditions of temperature extraction reactive process that was 40oc, reaction time of 30 minutes, 0. In this study, an hptlc densitometric method was developed for the determination of eugenol in above mentioned five spices as cloves dalchini,tejpatta,nutmeg and tulsi.
Hplc uv method development and quantification of eugenol from methanolic extracts of some spices farhin inam, sujata deo, neha narkhede 97 table 1. For example, the derivative of eugenol, acetyl eugenol itself play important role in food flavorings. In this paper, the commonly used steam distillation procedure has been modified to use microwave extraction. The mae allows for extraction of the essential oil in a fraction.
Specifically, an unmodified household espresso machine is used to extract the essential oil components of cloves eugenol and acetyleugenol. To perform this experiment cloves and water were mixed together and heated by a heating mantle in a roundbottom flask. The majority of cloves are used by kretek cigarette manufacturers in indonesia and only about 10 % for other purposes such as folk medicine, food flavouring, food preservatives, fragrance and pharmaceuticals. Pdf supercritical co2 extraction of essential oil from. This plant represents one of the richest source of phenolic compounds such as eugenol, eugenol acetate and. Isolation of eugenol report ramsey padilla jane erwin 03292012 introduction.
Isolation of methyl eugenol from cloves leaf oil using acid and based chemicals reactions has been done. Extraction of eugenol from cloves thermo scientific. In the study of isolation eugenol from clove oil, there is no data on the reaction temperature used and rkstraksi time used is relatively long. This week we will use steam distillation to isolate clove oil eugenol from cloves and determine how effective the technique is.
Microwave extraction of eugenol from cloves world journal of. Isolation of eugenol from cloves lab report free essays. These functional groups provide the basis for simple chemical tests used to characterize the clove oil. Characterization of the isolated clove oil eugenol contains a carbon. Steam distillation of a volatile oil from cloves purpose. Eugenol is a substituted methoxy phenol compound, a compound structurally similar to vanillin, but with an allylic functional group in place of the aldehyde in the. Essay about extraction of eugenol from cloves oil 994. Isolation of eugenol from cloves by steam distillation and. The boiling point of eugenol, an oil found in cloves, is 248 c, but it can be isolated at a lower temperature by performing a codistillation with water, this process is. Extraction of eugenol from cloves using an unmodified. A mixture of the essential oils, eugenol and acetyleugenol, will be steam distilled from cloves. Methyl eugenol produced from this research according to market needs with a minimum purity of 98. Eugenol, the main ingredient in clove oil, is a familiar fragrance in many dental offices as it is often mixed into a paste and used in dentistry as a local antiseptic.
Oil is often a water immiscible liquid that is separated by distillation. These compounds are isolated from aqueous distillate by extraction into dichloromethane. The most common method for the isolation of eugenol is steam distillation. To characterize eugenol and natural product by gas chromatography spectroscopy gcms and ir spectroscopy. The extraction of eugenol from cloves is a frequent experiment in organic chemistry labs at the sophomore level. The allylic chain gives eugenol its characteristic strong odor. Afterwards, the essential oils are mixed with 3% solution of sodium or potassium hydroxide for the extraction of eugenol. Various extraction methods have been practiced globally for the. Eugenol extraction from cloves using steam distillation the goal of. Cloves distinctive taste is said to carry the flavor of eugenol 5, which makes sense given the consistently high concentration of eugenol in cloves, which is on the order of 7289% in the extract 67. Introduction essential oils are the volatile components associated with the aromas of many plants.
To isolate eugenol from tea through distillation and rotary evaporation learn how to dry liquidsorganic solutions using a drying agent identify relevant parts of the structure of eugenol that result in its classification prelaboratory work. Prominent sources of eugenol are clove, cinnamon, tulsi and pepper. Extraction and isolation of eugenol the most common method for the isolation of eugenol is steam distillation. The experiment employs microwaveassisted extraction mae of the essential oil from whole cloves using aqueous ethanol and the subsequent isolation of eugenol by acidbase extraction. As a conclusion, this method is extremely simple and inexpensive for isolation of methyl eugenol from cloves leaf oil. Isolation of eugenol from cloves by julia b on prezi. Isolation of oil of clove and separation of eugenol and. Isolation of eugenol from cloves cloves water organic alayer a distill b extract wch 2cl 2 3 times e add c onc. Isolation of eugenol from cloves name justin arthur student lab partner witness 1. Eugenol is used in perfumery, for the manufacture of synthetic vanillin, as a dental topical analgesic, and as an antiseptic. Eugenol was synthesis from cloves leaf oil using sodium hydroxide naoh 5% and sulphuric acid h2so4 5% at ph 3 step 2. Background the boiling point of eugenol, an oil found in cloves, is 248 c, but it can be isolated at a.
Eugenol extraction from cloves using steam distillation. Cloves leaf oil was isolation from cloves leaf using steam distillation step 1. The spices cloves, dalchini, tejpatta,nutmeg and tulsi all of them contain an active component eugenol which can be helpful in preparation of various medicines. The isolation and biological testing of eugenol from clove oil. Eugenol is the main ingredient of the essential oil that can be isolated from the spice clove. Eugenol is used in germicides, perfumes, and mouthwashes, in. Microwaveassisted isolation of eugenol from cloves. To isolate acetyleugenol from clove seed and characterize it using ir. Cloves are strongly pungent owing to eugenol, which is extracted by distillation to yield oil of cloves. Hcl discare aqueous layer combined organic layers disare distillation flask clove residue collection flask distillate eugenol eug. Steam distillation allows mixtures of organic substances to distill and form a compound known as a distillate. Saponification is the process of making soaps from fats and a base. A greener undergraduate experiment for the organic chemistry lab. Isolation of eugenol from cloves abstract the experiment conducted, isolation of eugenol from cloves, is the extraction of the essential oil.
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